After a consistency check by the Kappa method, it was found that the results of mtDNA sequence had a high consistency in different tissues. The heteroplasmy positions and the mutation positions on HVâ… region were verified by Sanger sequencing. Different tissues in one individual had heteroplasmy difference. Six unrelated individuals belonged to 6 different haplotypes. The whole genome sequence of mtDNA from all samples were amplified successfully. Sanger sequencing was used to determine the heteroplasmy positions and the mutation positions on HVâ… region. #Marc Antoine torrent torrentWhole genome sequencing of mtDNA was performed using Ion Torrent PGMâ„¢ platform. #Marc Antoine torrent plusLibraries were constructed with Ion Shearâ„¢ Plus Reagents kit and Ion Plus Fragment Library kit. Amplification of whole genome sequence of mtDNA was performed by 4 pairs of primer. Samples were collected from 6 unrelated individuals by forensic postmortem examination, including chest blood, hair, costicartilage, nail, skeletal muscle and oral epithelium. To analyze and detect the whole genome sequence of human mitochondrial DNA (mtDNA) by Ion Torrent PGMâ„¢ platform and to study the differences of mtDNA sequence in different tissues. Ĭao, Y Zou, K N Huang, J P Ma, K Ping, Y Thus, this study indicates the utility of a cost and time efficient tool such as Ion Torrent sequencing to screen cancer mutations for the development of personalized cancer therapy. The sequencing analysis revealed a high occurrence of mutations along the TP53 locus (9.7%) in our sample set. We sequenced 737 loci from 45 cancer-related genes in 238 human gastric adenocarcinoma samples using the Ion Torrent Ampliseq Cancer Panel. In this study, we aim to identify genetic mutations in the genes which are targeted by drugs in clinical use or are under development in individual human gastric adenocarcinoma samples using Ion Torrent sequencing. Recent advances in new next-generation DNA sequencing technologies, such as the semiconductor-based Ion Torrent sequencing platform, makes DNA sequencing cheaper, faster, and more reliable. Until recently, identifying genetic mutations on an individual basis by DNA sequencing remained a daunting task. Since unique mutations have been observed in individual human cancer samples, identification and characterization of the molecular alterations underlying individual gastric adenocarcinomas is a critical step for developing more effective, personalized therapies. Gastric adenocarcinoma, the most common type of gastric cancer, is heterogeneous and its incidence and cause varies widely with geographical regions, gender, ethnicity, and diet. Gastric cancer is the one of the major causes of cancer-related death, especially in Asia. Xu, Zhi Huo, Xinying Ye, Hua Tang, Chuanning Nandakumar, Vijayalakshmi Lou, Feng Zhang, Dandan Dong, Haichao Sun, Hong Jiang, Shouwen Zhang, Guangchun Liu, Zhiyuan Dong, Zhishou Guo, Baishuai He, Yan Yan, Chaowei Wang, Lu Su, Ziyi Li, Yangyang Gu, Dongying Zhang, Xiaojing Wu, Xiaomin Wei, Xiaowei Hong, Lingzhi Zhang, Yangmei Yang, Jinsong Gong, Yonglin Tang, Cuiju Jones, Lindsey Huang, Xue F Chen, Si-Yi Chen, Jinfei Genetic mutation analysis of human gastric adenocarcinomas using ion torrent sequencing platform.
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